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Invitrogen™ TOP10 Electrocomp™ Kit
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.
Brand: Invitrogen™ C66411
This item is not returnable.
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Description
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids.
The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG
Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.
Specifications
| Electrocompetent Cells | |
| Yes (endA1) | |
| High Efficiency (>1 x 1010 cfu/μg) | |
| Yes (Streptomycin) | |
| Yes (lacZΔM15) | |
| May be used for plasmids >20 kb | |
| Yes (recA1) | |
| No | |
| Tube | |
| 10 x 100 μL |
| No | |
| Yes (mcrA) | |
| • 10 x 100 μL TOP10 Electrocomp E. coli • 50 μL pUC19 vector (10 pg/μL) Store at –80°C. • 15 mL S.O.C. Medium Store at room temperature. |
|
| Not suitable for cloning unstable DNA | |
| Low | |
| No | |
| Dry Ice | |
| E. coli (K12) | |
| Electrocomp™ |
For Research Use Only. Not for use in diagnostic procedures.