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Phasemaker™ TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System
This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.
Brand: Phasemaker™ A33253
596.70 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Includes: One bottle of TRIzol LS Reagent 200 mL and two bags of 100 Phasemaker Tubes
Description
This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.
The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.
• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments
Services
One year after receiving
Specifications
| • 200 mL TRIzol LS Reagent; room temperature • 200 Phasemaker Tubes; room temperature |
|
| 1 hr. | |
| Liquid Samples (e.g. Serum) | |
| Not High-throughput Compatible (Manual) | |
| 200 Preps | |
| 0.25 mL per 0.75 mL of TRIzol™ LS Reagent |
| Organic Extraction | |
| 20 to 50 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling | |
| Room Temperature | |
| Tissue: ≤10 μg per mg Cells: ≤15 μg per 106 cells |
For Research Use Only. Not for use in diagnostic procedures.