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Invitrogen™ Vivid™ CYP3A5 Green Screening Kit
Vivid™ CYP3A5 Green Screening Kit
Brand: Invitrogen™ P2969
Additional Details : Weight : 3.18000kg
Description
Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid™ CYP450 Screening Kits offer:Simple Mix and Read Kit Format
Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagents. The CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A5 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Unique Vivid™ Reagents for Bright Fluorescent Signals and Low Background
Vivid™ Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid™ DBOMF Substrate is the substrate included in this particular kit. This substrate yields a product that emits a green fluorescence.
Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid™ CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2μl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.
Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.
Specifications
| Vivid™ CYP3A5 Green Screening Kit, 300 Assays, Liquid, Green Color, 96-well Plate Format, Fluorescent Detection Method, CYP3A5 Detection Target, VIVID™ Green Label, Use With Microplate Reader, Used in Cell Viability, Proliferation and Function, Pharma and Biopharma, Protein Assays and Analysis, Target-Based ADME/Tox Assays | |
| Protein biology, cell analysis, cell viability, proliferation and function, cell metabolism, drug discovery and development, target-based adme and frasltox assays, biochemical ADME assays, cytochrome p450 inhibition adme assays, enzyme and protein activity assays, protein assays and analysis, cytochrome p450 activity | |
| –80°C | |
| 300 Assay(s) |
| Liquid | |
| Vivid™ CYP450 fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards, and an NADPH-regeneration system | |
| Vivid™ CYP3A5 Green Screening Kit | |
| 96-well plate |