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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide

Brand:  Invitrogen™ PV3174

Additional Details : Weight : 3.18000kg

Product Code. 12010176

  • 794.00 € / 800 reactions
Estimated Shipment: 06-08-2024
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For Research Use Only. All usage must comply with product instructions.


Description

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.
Specifications

Specifications

Z´-LYTE™
FRET
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Methylcoumarin
800 x 20 μL Assays
porcine pyruvate liver kinase (LRRASLG)
800 Reactions
End Point
Peptide-Based Substrate
AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5
Serine/Threonine Kinases
Serine/Threonine Kinase Assay
Biochemical phospho-specific activity (no antibody)
Kinase Assay Kit
384-well plate
405⁄465, 535
BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Dry Ice
PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA
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For Research Use Only. Not for use in diagnostic procedures.