Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 272 results

Gibco™ PBS, pH 7.2

PBS, pH 7.2

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Specifications

Dilution	1 X
Content And Storage	15°to 30°C
Additive	No Sodium Pyruvate
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.2
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	280 to 320 mOsm/kg

Gibco™ PBS (10X), pH 7.4

PBS (10X), pH 7.4

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Pricing and Availability
Specifications

Regulatory Status	For in-vitro diagnostic use
Dilution	10 X
Content And Storage	15°to 30°C
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Capacity	500 mL
pH	7.3 to 7.5
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3100 mOsm/kg

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Gibco™ PBS (10X), pH 7.2

PBS (10X), pH 7.2

Pricing and Availability
Specifications

Regulatory Status	For in-vitro diagnostic use
Dilution	10 X
Content And Storage	15°to 30°C
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Capacity	500 mL
pH	7.1 to 7.3
Shelf Life	36 Month(s)
For Use With (Application)	Mammalian Cell Culture
Osmolality	2800 to 3200 mOsm/kg

Gibco™ PBS, pH 7.4 (flow cytometry grade)

Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry and as a cell culture reagent.

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Medicago Phosphate Buffered Saline Tablets, pH 7.4

Specifically developed for immunological and microbiological laboratories. Medicago Phosphate Buffered Saline Tablets, pH 7.4 are provided as pre-weighed tablets with a final volume of 500 or 1000ml per tablet.

Pricing and Availability
Specifications

Content And Storage	25°C
Form	Tablet
Color	White
pH	7.4
Grade	Analytical Grade
For Use With (Application)	Immunoassays, tissue and cell culture procedures, immuno-histochemical procedures, microbiological procedures, and sample dilution

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

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Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Dilution	10 X
Content And Storage	Store at room temperature.
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.4
Product Line	Ambion™

Gibco™ PBS, pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

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Pricing and Availability
Specifications

Shipping Condition	Ambient
Dilution	1 X
Content And Storage	15°C to 30°C
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.3 to 7.5
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
For Use With (Application)	Mammalian Cell Culture
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Osmolality	280 - 315 mOsm/kg

Gibco™ PBS, pH 7.4

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

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Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Form	Liquid
Color	Clear
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
For Use With (Application)	Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Osmolality	280 - 315 mOsm/kg
Dilution	1 X
Additive	No Sodium Pyruvate
Product Type	Phosphate Buffered Saline (PBS)
Classification	Animal Origin-Free
pH	7.4
Solution Type	Phosphate Buffered Saline
Product Line	Gibco™

Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4

Non-toxic to most cells and is used in biological research.

Pricing and Availability
Specifications

Dilution	10 X
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Color	Colorless
pH	7.4

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 6.8

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

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Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

Thermo Scientific Chemicals Glycine, 0.2M buffer soln., pH 2.5

CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.07 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N IUPAC Name: 2-aminoacetic acid SMILES: NCC(O)=O

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Specifications

CAS	56-40-6
Molecular Weight (g/mol)	75.07
MDL Number	MFCD00008131
SMILES	NCC(O)=O
IUPAC Name	2-aminoacetic acid
InChI Key	DHMQDGOQFOQNFH-UHFFFAOYSA-N
Molecular Formula	C2H5NO2

Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 6.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

Pricing and Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
MDL Number	MFCD00283848
SMILES	C1COCCN1CCS(=O)(=O)O.O
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing and Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00132476
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Life Science Buffers

Life Science Buffers

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Gibco™ PBS, pH 7.2 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS (10X), pH 7.2 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.4 Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Gibco™ PBS, pH 7.2

Gibco™ PBS (10X), pH 7.4

Gibco™ PBS (10X), pH 7.2

Gibco™ PBS, pH 7.4

Gibco™ PBS, pH 7.4