Test
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocin™ (ZeoR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPO™ |
GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Shipping Condition | Dry Ice |
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Content And Storage | Includes master mix, positive control, and water and accommodates the use of your own competent cells. |
Workflow Step | DNA Assembly |
Number of Fragments | Up to 6 fragments |
Product Type | HiFi Master Mix |
Control Type | Positive Control |
Cloning Method | Seamless cloning |
For Use With (Application) | Seamless Cloning & Genetic Assembly |
Product Line | GENEART™ |
The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 μL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs.
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Format | 12-well Strips,8-well Strip,96- or 384-well Plate |
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Product Type | Sequencing Buffer |
For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
Technique | Fluorescent Dye Terminator Sequencing |
Product Line | BigDye™ Terminator |
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.
Shipping Condition | Room Temperature |
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Buffer | Sequencing Buffer |
Content And Storage | Store at 2°C to 8°C. |
Form | Liquid |
Concentration | 5X |
For Use With (Application) | Standard Sequencing |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPO™-TA |
Product Line | TOPO™ |
The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.
Delivers simple, 5-minute cloning and access to Gateway recombination cloning vector platform
Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System