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DMEM-F-12 Cell Culture Media

DMEM-F-12 Cell Culture Media

DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.
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1 – 15 of 27 results
1

Gibco™ DMEM/F-12, no glutamine
GREENER_CHOICE

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Shelf Life 12 Months
2

Gibco™ Advanced DMEM/F-12
GREENER_CHOICE

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Promotions are available
Shipping Condition Room Temperature
Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Reduced Serum
3

Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Standard Serum Supplementation
4

Gibco™ DMEM/F-12, HEPES
GREENER_CHOICE

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
5

Gibco™ DMEM/F-12, GlutaMAX™ supplement
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Promotions are available
Sterilization Method Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Form Liquid
Without Additives No HEPES
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1 X
Sterility Sterile-filtered
6

Gibco™ BenchStable™ DMEM/F12
GREENER_CHOICE

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Shipping Condition Room Temperature
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Store at room temperature. Protect from light.
Form Liquid
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 9001: 2015 standard
Green Features Energy efficient, Fewer resources used, Sustainable packaging
Concentration 1 X
Sterility Sterile-filtered
With Additives High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line BenchStable™
Serum Level Standard Serum Supplementation
7

Gibco™ KnockOut™ DMEM/F-12
GREENER_CHOICE

KnockOut™ DMEM/F-12

EDGE
8

Gibco™ DMEM/F-12, HEPES
GREENER_CHOICE

DMEM/F-12, HEPES

EDGE
Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With L-glutamine, HEPES, Phenol Red, Sodium Pyruvate, 6.9 to 7.3 pH, 12 Months Shelf Life, 270 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
pH 6.9 to 7.3
Shelf Life 12 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
9

Gibco™ DMEM/F-12
GREENER_CHOICE

DMEM/F-12

EDGE
10

Gibco™ CTS™ KnockOut™ DMEM/F-12
GREENER_CHOICE

CTS KnockOut DMEM/F-12 CTS is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

11

Gibco™ DMEM/F-12, no phenol red
GREENER_CHOICE

DMEM/F-12, no phenol red

EDGE
12

Gibco™ DMEM/F-12, GlutaMAX™ Supplement
GREENER_CHOICE

DMEM/F-12, GlutaMAX™ Supplement

Content And Storage 2 to 8°C
Description DMEM/F-12 Medium, 500mL, 1 X Concentration, Liquid Form, With GlutaMAX™, Phenol Red, Sodium Pyruvate, 6.8 to 7.2 pH, 12 Months Shelf Life, 290 to 330mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture
With Additives GlutaMAX,Phenol Red,Sodium Pyruvate
Product Type DMEM/F-12 Medium
Form Liquid
Without Additives No HEPES
pH 6.8 to 7.2
Shelf Life 12 Months
Concentration 1 X
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture
13

Gibco™ DMEM/F-12, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

14

Gibco™ DMEM/F-12, HEPES, no phenol red
GREENER_CHOICE

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

EDGE
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Without Additives No Phenol Red,No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Concentration 1X
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,HEPES
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Classification Animal Origin-free
Shelf Life 12 Months From Date of Manufacture
Product Line Gibco™
Serum Level Standard Serum Supplementation
15

Gibco™ DMEM/F-12, powder

DMEM/F-12, powder

Form Powder
Without Additives No HEPES,No Sodium Bicarbonate
pH 6.2 to 6.6
Shelf Life 36 Months
Certifications/Compliance ISO 13485 standards, FDA
For Use With (Application) Mammalian cell culture