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  7. 16325342

Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

Brand:  Applied Biosystems™ 4458687

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Product Code. 16325342

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Includes: This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions
This item is not returnable. View return policy

Description

Description

  • A streamlined, simple procedure
  • Performance of all sequencing steps in a single reaction tube
  • Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
  • Substantial time savings versus previous workflows

Order Info

1 Kit

TRUSTED_SUSTAINABILITY
Specifications

Specifications

This kit contains BigDye™ Direct PCR Master Mix, BigDye™ Direct Sequencing Master Mix, BigDye™ Direct M13 Fwd Primer, BigDye™ Direct M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 100 reactions.
8-well Strip, 96-well Plate
Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
BigDye™
100 reactions
Fluorescent Dye Terminator Sequencing
BigDye™ Direct Cycle Sequencing Kit, 100 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler
3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer
100
Direct Cycle Sequencing Kit
Dry Ice
BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)
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For Research Use Only. Not for use in diagnostic procedures.