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Thermo Scientific™ CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line
The CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression, along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs.
Brand: Thermo Scientific™ K1468
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Description
The CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline-induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.
Specifications
| Cell-based beta lactamase reporter gene | |
| CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. | |
| Fluorescence | |
| CellSensor™, T-REx™ | |
| HeLa | |
| Cell Line | |
| CellSensor™ |
| Dividing Cells | |
| CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line, 1Vial, ∽ 2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, HeLa Cell Line, Fluorescent Detection Method, Kinases Druggable Target, Adherent Growth Properties, Liquid Nitrogen Storage | |
| Cellular imaging, pharma and biopharma, cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, target and lead identification and validation, pathway biology, cellular pathway analysis assays, cellsensor™ cellular pathway assays | |
| 1 Vial | |
| IR, IGF1R, PI3K, AKT1, FOXO3, PI3K/AKT/FOXO3 pathway | |
| HeLa | |
| Kinases |
For Research Use Only. Not for use in diagnostic procedures.