Learn More
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Brand: Thermo Scientific™ EN0521
53.25 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.
Description
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.
The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.
- In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
- In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides
Highlights
- Recombinant enzyme
- Purified from non-animal host with a lower level of intrinsic RNases
Applications
- Preparation of DNA-free RNA
- Removal of template DNA followingin vitrotranscription
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- DNA labeling by nick-translation in conjunction with DNA Polymerase I
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
Note
DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifications
1 U/μL | |
Reaction Buffer | |
DNase I RNase Free |
DNase | |
1,000 units |
Product Suggestions
Customers who viewed this item also viewed
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Special Offers
-
Multiple Manufacturers
New Offer Shop Now
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only. Not for use in diagnostic procedures.