missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Brand:  Thermo Scientific™ EN0521

53.25 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.


Product Code. 10649890

  • 75.00 € / 1000 units

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.

  • In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
  • In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases

Applications

  • Preparation of DNA-free RNA
  • Removal of template DNA followingin vitrotranscription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 U/μL
Reaction Buffer
DNase I RNase Free
DNase
1,000 units
Product Suggestions

Product Suggestions

Videos
SDS
Documents Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number2797506 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEN0521
Lot Number2826726 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEN0521
Lot Number2838992 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEN0521
Lot Number2841107 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEN0521
Lot Number2859648 Certificate TypeCertificate of Analysis Date28/01/2025 Catalog NumberEN0521
Special Offers

Special Offers

  • Multiple Manufacturers
    Fall in love with big savings
    Promo Code: 24107

    Expires2025-03-31

    New Offer
    Shop Now
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Thermo Scientific™ DNase I, RNase-free (1 U/μL) > 1U/uL; 1000U

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.