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Thermo Scientific™ GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Cells

GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.

Brand: Thermo Scientific™ K1708

Product Code. 10592754

16470.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

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Description

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GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cells (Cat. No. K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.

The GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of apomorphine. In addition, GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The D2 receptor is found primarily in the brain. There are two forms of D2 which are created by alternate splicing: a short form and a long form. This assay uses the long form, although little difference in ligand binding or signaling has been seen between the two forms.

Specifications

Accession Number	NM_000795
Cell State	Dividing Cells
Description	GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application)	FRET
Includes	GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells, GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Assay Protocol, Certificate of Analysis
Product Line	GeneBLAzer™
Reporter Gene	BLA (Beta-Lactamase)
Target Entry	DRD2, D2
Cell Line	CHO-K1
Druggable Target	GPCR

Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells • GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis The GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	D2
Population Status	Single Cell Clone Origin
Quantity	2 x 10⁶ Cells
Sample Type	CHO-K1
Type	CHO-K1 Cells
System Type	GeneBLAzer™

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Documents

Product Information

Manuals

User Guide: GeneBLAzer D2-Gqo5-NFAT-bla CHO‑K1 Cell–Based Assay Version: 2.0 File size: 413 kB

GeneBLAzer® D2-Gqo5 NFAT-bla CHO-K1 Cell Line Validation Packet Version: version 1.1 File size: 174.6 kB

Certificates

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Lot Number

1 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number36804	Certificate TypeCertificate of Analysis	Date16/04/2017	Catalog NumberK1708

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