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Thermo Scientific™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

Brand: Thermo Scientific™ K1689B

Product Code. 10145793

10380.00 € / Each

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Estimated Shipment: 03-01-2025
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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER beta-UAS-bla GripTite™293 cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

Specifications

Cell State	Dividing Cells
Detection Method	Fluorescence, Fluorescent
For Use With (Application)	Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Includes	GeneBLAzer™ ER beta -UAS-bla GripTite™ cells Includes: ER beta-UAS-bla GripTite™ cells, Protocol, Certificate of Analysis
Product Line	GeneBLAzer™
Product Type	GripTite Cells
Reporter Gene	BLA (Beta-Lactamase)
Type	Cell Line

Content And Storage	ER beta-UAS-bla GriptTite™ cells (K1091) Shipping: Dry Ice Storage: -80°C (liquid nitrogen), Liquid nitrogen, room temperature
Description	GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells, 2 x 106 Cells, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Druggable Target	Nuclear Receptors
No. of Cells	2 x 10⁶ Cells
Quantity	2 x 106 Cells, 2 x 10⁶ cells
Sample Type	GripTite™ 293
Shipping Condition	Room Temperature

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Thermo Scientific™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

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