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Thermo Scientific™ GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

Brand: Thermo Scientific™ K1749

Product Code. 10686404

16470.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession No. NP_037477) stably integrated into the CellSensor™ NFAT-bla Freestyle HEK 293F cell line. CellSensor™ NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC₅₀ concentrations of dDAVP. In addition, GeneBLAzer™ GeneBLAzer™ T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Specifications

Accession Number	NM_013345
Cell State	Dividing Cells
Detection Method	Fluorescence
Gene Symbol	G2A
Population Status	Single Cell Clone Origin
Quantity	2 x 10⁶ Cells
Target Entry	GPR132, G2A
Cell Line	FreeStyle 293-F Cells
Druggable Target	GPCR

Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells • GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol • Certificate of Analysis The GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
For Use With (Application)	FRET
Includes	GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F cells, GeneBLAzer™ TREx™-G2A-NFAT-bla Freestyle293F Assay Protocol, Certificate of Analysis
Product Line	GeneBLAzer™
Reporter Gene	BLA (Beta-Lactamase)
Type	Dividing Cells
System Type	GeneBLAzer™

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Thermo Scientific™ GeneBLAzer™ T-REx™-G2A-NFAT-bla FreeStyle™ 293F Cells

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