missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10461025

Thermo Scientific™ GeneBLAzer™ VDR DA Assay Kit

GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1417

Product Code. 10461025

  • 1538.00 € / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both VDR DA cells and VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Cell-based beta lactamase reporter gene
Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
• VDR HEK 293T DA cells (K1417A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Nuclear Receptors
VDR
TO901317
1 Plate
VDR, NR1I1
293T (HEK)
Nuclear Receptors
Division-Arrested Cells
Fluorescence
7421
VDR HEK 293T DA cells (K1417A) LiveBLAzer™-FRET B/G Loading Kit, 70 μg
GeneBLAzer™
BLA (Beta-Lactamase)
Division-Arrested Cells Assay Kit
GeneBLAzer™
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

1 results found
Lot Number Certificate Type Date Catalog Number
Lot Number885119 Certificate TypeCertificate of Analysis Date15/04/2017 Catalog NumberK1417
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Thermo Scientific™ GeneBLAzer™ VDR DA Assay Kit > 1 Plate

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.