missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ LanthaScreen™ STAT5 TF-1 Cell Line

LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context.

Brand:  Thermo Scientific™ K1598

Product Code. 10583284

  • 11390.00 € / Each
Estimated Shipment: 16-01-2025
to see stock.
Explore more special offers
Add to basket

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. In hematopoeitic cells, the JAK2/STAT5 signaling pathway plays an essential role in blood cell formation in response to cytokines such as GM-CSF, IL-3, and EPO. In this pathway, binding of these cytokines to their respective cell surface receptors results in the activation of JAK2, which in turn phospho-activates STAT5 proteins at specific tyrosine residues (Tyr-694/699).

LanthaScreen™-STAT5 TF-1 is a human hematopoeitic cell line which constitutively expresses GFP-STAT5 fusion proteins. The JAK2/STAT5 signaling pathway is known to be functionally intact in this cell line, therefore the GFP-STAT5 fusion protein serves as a substrate for the inducible phosphorylation by JAK2. Using this cell line, a homogenous immuno-assay has been developed in which the phosphorylation state of GFP-STAT5 is detected in cell lysates using a LanthaScreen™ Terbium-anti-mouse and anti-phospho STAT5 [pTyr694/699] antibody pair, in a time-resolved FRET (TR-FRET) readout. GFP-STAT5 Lentivirus was transduced into TF-1 cells followed by selection with Blasticidine.

This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker, and has been screened for the constitutive expression of GFP-STAT5 fusion protein. Using a lytic TR-FRET immuno-assay, this cell line is validated for EC50 and Z' under optimized conditions using GM-CSF as an agonist for JAK2 -mediated GFP-STAT5 phosphorylation. This assay has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and lysis/equilibration time.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

IL-3, EPO
Cell-based terbium phospho-specific activity (stable)
LanthaScreen™ STAT5 TF-1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Fluorescence
STAT5A
GM-CSF
1 Vial
GFP (EmGFP)
STAT5
TF-1
Signaling Pathway
JAK Inhibitor
Dividing Cells
LanthaScreen™ STAT5 TF-1 Cell Line, 1Vial, Fluorescent Detection Method, Kinases, Signaling Pathway Druggable Target, LanthaScreen™ System, End Point Readout, Liquid nitrogen Storage
Pharma and biopharma, drug discovery and development, target and lead identification and validation, kinase biology, kinase pathway analysis, pathway biology, cellular pathway analysis assays, cellSensor™ cellular pathway assays
GM-CSF (EC50 = 0.33 ng/mL)
LanthaScreen™
TR-FRET
TF-1
Cell Line
LanthaScreen™
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.