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Invitrogen™ LysoLight™ Deep Red Antibody Labeling Kits
LysoLight™ Deep Red Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight™ Deep Red dye. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.
Brand: Invitrogen™ L36003
This item is not returnable.
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Description
LysoLight Deep Red Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight Deep Red has little to no observable background fluorescence, and only upon localization to the protease rich environment of the Lysosome, does LysoLight become activated and brightly fluoresce.
The labeling protocol provided in the kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight Deep Red dye, buffers, and purification columns, to facilitate the antibody labeling process.
The LysoLight Deep Red SDP ester contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 µg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive, SDP ester form of the LysoLight Deep Red dye for labeling either 100 µg or 1 mg of IgG.
Specifications
| Deep Red | |
| Fluorescence | |
| Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument | |
| Enhanced stability | |
| 3 x 100 μg | |
| 650/668 nm |
| LysoLight™ Deep Red Amine Reactive Dye | |
| Antibody and Protein Labeling | |
| Reactive dye | |
| LysoLight™ | |
| DMSO (Dimethylsulfoxide) |
For Research Use Only.