Invitrogen™ LysoLight™ Deep Red Antibody Labeling Kits

Description

LysoLight Deep Red Antibody Labeling Kits provide a powerful tool for visualizing the lysosomal localization and catabolic degradation of antibodies and proteins. These kits utilize a unique fluorophore that is only active upon enzymatic cleavage by the lysosomal specific enzyme, Cathepsin B. Outside of the lysosome, LysoLight Deep Red has little to no observable background fluorescence, and only upon localization to the protease rich environment of the Lysosome, does LysoLight become activated and brightly fluoresce.

The labeling protocol provided in the kits involves a simple and rapid procedure, ensuring minimal hands-on time and reliable results. The kits include all necessary reagents, including the LysoLight Deep Red dye, buffers, and purification columns, to facilitate the antibody labeling process.

The LysoLight Deep Red SDP ester contained in these kits readily reacts with lysine residues in antibodies or proteins to yield a covalently attached fluorogenic sensor. The spin columns included in the kits are used for purifying the labeled antibody from excess dye with yields of 70–95%. The kits contain sufficient reagents for three labeling reactions of 100 µg or 1 mg of antibody. The user guide describes a general protocol for using the amine-reactive, SDP ester form of the LysoLight Deep Red dye for labeling either 100 µg or 1 mg of IgG.