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Thermo Scientific™ Tango™ GALR2-bla U2OS Cells

Brand: Thermo Scientific™ K1811

Product Code. 10064004

13050.00 € / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

The Tango™ GALR2-bla U2OS cells contain the human Galanin Receptor 2 (GALR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GALR2-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Galanin (1-30). In addition, Tango™ GALR2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

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Specifications

Accession Number	NM_003857
Cell State	Dividing Cells
Cytoplasmic Tail	Native Tail
For Use With (Application)	FRET
Human Protein Class	G-Protein Coupled Receptor (GPCR)
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ Cells
Target Entry	GALR2
Cell Line	U2OS
Druggable Target	GPCR

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ GALR2-bla U2OS cells • Tango™ GALR2-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ GALR2-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	GALR2
Includes	GALR2-bla U2OS cells, GALR2-bla U2OS Assay Protocol, Certificate of Analysis
Product Line	GeneBLAzer™, Tango™
Readout	End Point
Type	Dividing Cells
System Type	Tango™

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Thermo Scientific™ Tango™ GALR2-bla U2OS Cells

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